Deoxyribonucleic Acid (DNA) Isolation of Different Parts of Rice Plant (Oriza Sativa) using Modified CTAB Method

Abstract

This study focused on the isolation of DNA from leaves, stem, roots and rice hull of NSIC Rc222. Comparison of the quality and quantity is also done to determine if there is no significant difference among the samples.The findings are as follows:1. Level of concentration of DNA in the leaves, roots, stem and rice hull. The higher concentration of DNA was found on the leaves with a mean value of 3080.80, followed by the stem (2004.43), then the root (1318.77) and lastly 170.67 mean values for rice hull.2.Level of purity of DNA in the leaves, roots, stem and rice hull.In terms of purity of DNA, both 1.96 were obtained from rice hull and root.A purity of 2.16 from the stem and a higher purity were coming from the leaves with a mean value of 2.18.3. Comparison of the Concentration level of DNAA result of there is a significant difference between the concentrations of DNA of four samples was found.4. Comparison of the Purity level of DN. There is a significant difference between the purity of DNA of four samples was the result of the study even though that all samples were in the ranged of good quality DNA.In the light of the findings of the study, the following conclusions are drawn:1. Researcher practice is to isolate DNA from the leaves because it was proven from past studies DNA is concentrated on the leaves and it is also found in this study. From this study, it was also found that DNA can be also isolate from the root, stem and rice hull.2. DNA from the root, stem and rice hull can had a good quality of purity that can be used to some applications.3. DNA quality and quantity of roots stem and rice hull of rice plant was not comparable with the quality and quantity of the leaves. Based from the results of the study and conclusions, the following recommendations are given: Other method of DNA extraction can be employed. Aside from stem, root and rice hull and other part of the rice plant can be used. Carbonized parts of the plants can be used as samples. Utilize another variety of rice plant or other crops.