Brine Shrimp Lethality Assay (Bsla) of the Aqueous Extract of Melothria Pendula Found in Rosario, Cavite

Abstract

INTRODUCTION

Creeping Cucumber (Melothria Pendula) is one of the traditional herbal medicine that definitely contains toxic properties. According to Lira et al. (1998), it contains 12.6% protein, 16.30% of fiber, and lastly 56.8% of carbohydrates. It is said to be good in ruminants. In addition, chemical bromatologic said that Melothria Pendula carries out water and protein, has a pleasantly sweet flavor, and, most importantly, it can be an additional nutria alternative.

METHODS

The researchers used the Brine Shrimp Lethality Assay (BSLA) to determine the cytotoxic effects of Melothria Pendula extract. Plant extract was impregnated with 5 levels of concentration -0ppm, 250ppm, 500ppm, 750ppm, 1000ppm. The researchers counted the number of surviving shrimps and recorded after 24 hours.

RESULTS

Through the Brine Shrimp Lethality Assay (BSLA) of the Aqueous Extract of Melothria Pendula found in Rosario, Cavite, the researchers finally identified that Melothria Pendula is a toxic plant. Furthermore, the researchers concluded that it has a significant effect on the determination of the cytotoxic effects of the Melothria Pendula extract.

DISCUSSIONS

Through the Brine Shrimp Lethality Assay (BSLA) of the Aqueous Extract of Melothria Pendula found in Rosario, Cavite, the researchers finally identified that Melothria Pendula is a toxic plant. Furthermore, the researchers concluded that it has a significant effect on the determination of the cytotoxic effects of the Melothria Pendula extract.