Teratogenic Effects of Newly Domesticated Lentinus strigosus, BIL1324 on Zebrafish (Danio rerio) Embryos

Abstract

Lentinus strigosus, BIL1324 or locally known as Kabuteng Balbon, is a naturally occurring, wood-rotting mushroom found on fallen logs. With the aim to assess its potential as a source of a toxic compound to find ‘nutraceutical’ that could help in developing natural anticancer drugs, this study highlighted the teratogenic effects of newly domesticated L. strigosus, BIL1324 on zebrafish (Danio rerio) as an animal model. Its functional components obtained through hot water extraction exhibited teratogenic effects on zebrafish embryos. The mushroom extract significantly lessens the hatchability of zebrafish eggs at 0.5% and higher concentrations. Embryos exposed at 0.5% -3% concentrations significantly showed developmental delay. Non-detachment of the tail bud, lack of eye bud at segmentation phase, and bend out the shape of the body of zebrafish at pharyngula period after 48 hours of exposure to mushroom extract and were recorded as growth-delay endpoints. The delayed development resulted to coagulation of embryos at earlier phase (3% treatment concentration) as developmental apical points impede the breakage of chorion before hatchability. It also found that the mushroom extract is dose and time-dependent. The results indicate that L. strigosus, BIL1324 contains bioactive components that induced teratogenic effects on zebrafish embryos.