Blood Coagulation Activity of Chitosan Extracted from bracket Fungi (Trametes sp.)on the Blood of Male Albino Mice

Abstract

The most widely recognised wellspring of chitosan are crustaceous shells; but, mushrooms and fungi are an elective hotspot for chitosan. This study aimed to determine the blood coagulation activity of chitosan extracted from Bracket Fungi (Trametes sp.).The chitosan was extracted from Bracket Fungi using alkaline and acid treatment, and it was placed in a rotary evaporator at 60 degrees angle for an hour. Thirty percent of concentrated extract underwent evaporation to be in a semi-solid state. 0.5 mL, 1.0 mL, and 1.5 mL of chitosan and 0.2 mL of distilled water (negative control) were placed on each vial. 0.2 mL of blood from male albino mice was introduced on each vial, one at a time. Two replicates were made for each set-up. Each set up was observed until the blood already coagulated. The data were recorded and analyzed using Duncan Multiple Range Test. The 1.50% of chitosan solution had the fastest coagulation time with an average time of 4.73 seconds, while the slowest set-up is the negative control with 23.63 seconds. Among the blood coagulation time for the chitosan solutions, 1.00% and 1.50% were significantly faster than the 0.50% chitosan solution. Likewise, the 1.00% and 1.50% chitosan solutions were significantly faster than the negative control. All the chitosan solution exhibited faster coagulation time compared to the negative control, thus, the results of this study showed that chitosan can induce blood coagulation activity.