Comparative Analysis of Paper Decomposition by Cellulase-producing Actinomycete Isolates

Authors

  • Yohanna P. Bugarin
  • Crystal Joyce T. Ameida
  • Micaella A. Javier

Keywords:

paper decomposition, actinomycete isolates

Abstract

Land pollution is a common problem caused by improper solid waste management in Metro Manila. Seventeen percent of this is contributed by paper due to minimal recycling practices. Researchershypothesisedwhether cellulase-producing Actinomyceteisolates could decompose paper by measuring the decomposition rate. Actinomycete isolates were tested for cellulase. Yeast Malt Broth and minimal media containing 0.5g of cellulose were steamed. YMB media was used to revive the isolates. Isolates were inoculated into the minimal media. The positive growth of spores was screened. Components of YMB media were steamed, and isolates from first screening were inoculated into the media. The broth was screened for the growth of isolates, and the positives were used for paper decomposition. These were tested into three replicates using both of isolates that grew in YMB and its counterpart in minimal media. The observation lasted for 50 days. Decomposition rate was measured afterwards. One-way ANOVA and two-way ANOVA were used to analyse data. It showed that F-value (4.36682) was greater than the F-critical value (3.47805) in one-way ANOVA.VYMA 6 YMB had the greatest decomposition rate of 25.67%, making it the most effective based on the mean. The research showed that there was a significant difference in the decomposition ability of all the setups with yeast malt broth. There was a significant interaction between the isolate used and the media used. This study showed that cellulase-producing isolates could decompose paper and the decomposition of the isolate was affected by the media it is in, whether minimal media or yeast malt broth.

Published

2018-05-18