Saba Banana (Musa acuminata x balbisiana) Peels as an Additive to Potato Dextrose Agar for Fungi Mycelium Growing
Keywords:
Saba, Potato Dextrose Agar, Fungi MyceliumAbstract
INTRODUCTION
Many culture medias have developed due to various needs of varying microorganism. It continued to improve throughout times. The researchers devised a way to make a new kind of culture media by adding an additive from saba banana. The researchers want to study the effects of additives to normal culture medias.
METHODS
Start by preparing the materials. Then a mixture of equal amounts of water and saba banana peels were blended. Then we prepared home-made PDA and mixed it with the banana peels that were puréed to make 3 experimental groups and 1 control. Then it was transferred to a sterile container and put in the autoclave. Then it is ready for the inoculation phase. To test the capability of the additive to PDA, the researchers decided to cultivate a mushroom culture of the Pleurotus florida specie. The researchers got the tissue of the said mushroom and inserted it to the sample. After inoculation, it is stored in a cool environment and left to grow for three weeks.
RESULTS
The results show that addition of the Saba banana peels has an effect on the physical properties, and to the effectiveness of the culture media. Only 2 of the experimental groups have managed to solidify as normal culture media would. The results of the experiment were obtained by getting the mycelial running rate of the culture.
DISCUSSIONS
As a conclusion, the most optimal quantity of Saba banana additive to PDA is in the ratio of 50-50. Although the group with 30 ml banana mixture and 10 ml PDA yields faster effects, the researchers also took into consideration the physical properties of the obtained culture media. The research showed that adding saba banana peels into PDA increases mycelia growth rate from two months to a span of two to three weeks.