Laurus nobilis (Bay Laurel) Leaves Extract Against the Growth of Two Gram-Positive Bacteria: Staphylococcus aureus and Bacillus subtilis

Abstract

INTRODUCTION

Gastroenteritis is an aggravation of the gastrointestinal tract including both the stomach and the small digestive tract. It is usually associated with food poisoning which may be bacterial or chemical in origin. This research study aims to determine the efficiency of the Laurus nobilis (Bay Laurel) extract as a potential substitute for Vancomycin in inhibiting the bacteria Staphylococcus aureus and Bacillus subtilis.

METHODS

Plant verification was done to authenticate and verify the plant. The fresh leaves were prepared and subjected to soxhlet extraction for the isolation of the essential oil. The different concentrations (25%, 50%, 75%, and 100%) of the isolated constituent, Vancomycin (positive control), distilled water (negative control) and 95% ethanol as controlled variable. The accomplished extract was subjected to an antibacterial test using several processes such as disc diffusion method and incubation. The zone of inhibition was measured using a metric ruler and analyzed using One-way ANOVA at .05 level of significance.

RESULTS

Results showed that the 100% concentration containing pure isolated extract exhibited the greatest zone of inhibition against Staphylococcus aureus and Bacillus subtilis.

DISCUSSIONS

The research study can serve as a proof or basis that Bay Laurel can inhibit Staphylococcus aureus and in Bacillus subtilis which will be a great help to experts and other researchers. This research can be applied in the field of pharmacy and medicine.