Effectivity of Laurus nobilis (Bay Laurel) Leaves Extract on the Growth of Two Gram-Positive Bacteria: Staphylococcus aureus and Baci

Abstract

INTRODUCTION

Gastroenteritis is an aggravation of the gastrointestinal tract including both the stomach and the small digestive tract. It is usually associated with food poisoning which may be of bacterial or chemical origin. This research study aimed to determine the efficiency of the Laurus nobilis (Bay Laurel) extract as a potential substitute for Vancomycin in inhibiting the bacteria Staphylococcusaureus and Bacillus subtilis.

METHODS

Plant verification was done to authenticate and verify the plant. The fresh leaves were prepared and subjected to Soxhlet extraction for the isolation of its essential oils. The different concentrations (25%, 50%, 75%, and 100%) of the isolated constituent were the following: Vancomycin (positive control), distilled water (negative control) and 95% ethanol as the controlled variable. The accomplished extract was subjected to an antibacterial test using several processes such as disc diffusion method and incubation. The zone of inhibition was measured using a metric ruler and analyzed using One-way ANOVA at .05 level of significance.

RESULTS

The 100% concentration containing the pure isolated extract exhibited the greatest zone of inhibition against Staphylococcus aureus and Bacillus subtilis.

DISCUSSIONS

The research study can serve as a basis that Bay Laurel can inhibit Staphylococcus aureus and in Bacillus subtilis which can be a great help to experts and other researchers. This research can be applied in the field of pharmacy and medicine.