Effect of Blue Green Algae (Cyanobacteria) on Lacatan Banana (Musa acuminata) Tissue Culture

Abstract

INTRODUCTION

Plant tissue culture is the technique of maintaining and growing cells, tissues or organs especially on an artificial medium in suitable containers under controlled environmental conditions. Banana is the most economically important fruit crop in the Philippines and the only locally grown fruit available all year-around. However, supply does not always meet this high demand, especially in Luzon. Lakatan, sold in Luzon, sometimes comes from Mindanao. This study was conducted to use blue-green algae homogenized crude extract as an alternative source of plant growth regulator on Lakatan banana tissue culture. It helped produce large quantities of banana in just a short time. This also contributed to the removal of harmful algae on bodies of water, specifically from the Sampaloc Lake found at San Pablo City, Laguna.

METHODS

Using forceps and scalpel, the banana shoots are planted on the culture media. The treatment are MS + 3 PDR BAP (sa3) ; MS + charcoal (sa0) ; MS without charcoal ; MS + filtered homogenized crude extract of blue-green algae ; MS + unfiltered homogenized crude extract of blue-green algae. There are a total number of 15 replicates containing 2 shoots for each test tube. The data gathered was statistically analyzed using one-way ANOVA to determine if there was a significant difference between the varying treatments.

RESULTS

On the 32nd days after planting, MS + Blue-green algae yield the most number of shoots, MS without charcoal has the highest length of shoots and MS + 3 PDR BAP has the greatest number of leaves. While on the 64th days after planting, MS + Blue-green algae yield the most number of shoots, MS +Blue-green algae have the highest length of shoots and MS + Blue-green algae have the greatest number of leaves.

DISCUSSIONS

Arriving at the statistical analysis of data, the researchers came up with the result that the MS + Blue-Green Algae homogenized crude extract (Treatment 4) is the best treatment for the production of shoots; that MS + charcoal (Treatment 2) is good for lengthening of plants while MS without charcoal (Treatment 3) and MS + 3 PDR BAP (Treatment 1) are good in production of leaves.