Comparative Analysis on the Anti-Angiogenic Activity Between Hugis-Manok and Pandan on the Chorioallantoic Membrane of the 10th Day Old Duck Embryo

Abstract

INTRODUCTION

The Philippine duck industry is dominated by balut or, partially hatched duck embryos, the production of which accounts for more than 75 percent of the total duck industry. Angiogenesis has become an important issue in fighting against the progress of cancer because it was thought that anti-angiogenic drugs prevent the growth of cancer cells by blocking the development of new blood vessels. There is a big challenge, therefore, to investigate plant extracts that have the potential to inhibit angiogenesis. These ideas motivated the researchers to conduct a study on the anti-angiogenic property of Pandan (Pandanus Odoratissimus L.) and Hugis Manok (Eclipta Prostata L.l) leaves extract.

METHODS

The two raw materials were collected within Imus and Bacoor City and left aside for 72 hours for air drying. The Pandan (Pandanus Odoratissimus L.) and Hugis Manok (Eclipta prostata L.l) were soaked and extracted for 48 hours. 42 (forty-two) eggs were cleaned. With the use of flashlight, the researchers looked for the Chorioallantoic membrane (CAM Assay) of the egg and injected the extracted materials using tuberculin syringe with different concentrations -100% pure extracts,75% leaves extract added with 25% distilled water, 50% leaves extracts added with 50% distilled water and 25% leaves extract added with 75% distilled water -to determine which concentration has the great potential in inhibiting the growth of blood vessels. Then eggs were incubated for 72 hours. After 72 hours the eggs were opened, blood vessels were counted and analyzed.

RESULTS

After three (3) days of incubation, the results revealed that Pandan (PandanusOdoratissimus L.) leaves extracts gave the lower mean number of blood vessels with 42.6. This means that Pandan (Pandanus Odoratissimus L.) has a higher potential in lowering the blood vessels count.

DISCUSSIONS

Based on the findings and results from the analysis of the study, it was revealed that Pandan (Pandanus Odoratissimus L. ) leaves extract contains the phytochemical components tannins, steroids, cardiac glycosides, saponins, flavonoids, alkaloids, and carbohydrates compared to Hugis Manok (Ecliptaprostate L.l). These phytochemicals are reported to have an anti-angiogenic effect, preventing the faster production of new blood vessels.