Comparative Analysis of Oregano (Origanum vulgare) and Guava (Psidium guajava L.) Leaves Extract as Bio Fungicide Against Fusarium oxysporum sp. lycopersici in Tomato

Abstract

INTRODUCTION

Fusarium wilt on tomatoes is caused by Fusarium oxysporum sp. lycopersici. The purpose of this study was to find alternative botanical extracts such as Oregano (Origanum vulgare) and guava (Psidium guajava L.) leaves as fungicide against the said fungi.

METHODS

The materials were gathered from different places, namely San Pablo City and Nagcarlan, Laguna. The infected tomatoes with the probability of having the disease were washed repeatedly using ethyl alcohol and distilled water. Afterward, sterilized paper towels were used for the process of drying until no signs of liquid are visible. Pieces of the sliced tomatoes were placed in a Petri plate containing PDA (Potato Dextrose Agar) and were left for the fungi to grow. The gathered leaves were pounded in mortar and pestle and were put in a rotary evaporator. Then, pounded leaves were put in sterilized bottles with solvent. Afterward, the bottles were set into a water shaker for 3 days with a temperature of 27-28 degrees. The matured fungi with the crude ethanolic extracts were observed for days to know which set-up showed inhibited growth of Fusarium oxysporum sp. lycopersici.

RESULTS

The researchers observed the results for two days, on the 3rd and 5th day of the experiment to test the inhibition. The results showed that oregano, guava, and the chemical Dithane M45 fungicide were able to inhibit the growth of fungi during the 3rd day while oregano was the only set-up that had inhibited the growth of fungi on the 5th day.

DISCUSSIONS

On Day 5, only the set-up of oregano had inhibited the growth, thereby making it the most effective out of all the treatment given in this study.