Preliminary Phytochemical Screening and In-vitro Evaluation of Inhibitory Activity of Cottonfruit (Sandoricum koetjape)Crude Ethanolic Fruit Peel Extract against Aeromonas hydrophila

Authors

  • Ma. Michelle Edlyn Mhae F. Alarcon

Keywords:

Phytochemical screening, Sandoricum koetjape, disc diffusion assay, Aeromonas hydrophila

Abstract

Bacterial infections are the major reasons for fish mortality in the aquaculture industry. Vaccines and antibiotics against specific fish pathogens have been developed with varying degree of success. However, the use of antibiotics and chemotherapeutics in intensive aquaculture has been widely criticized due to their negative impacts like an accumulation of drugs in fish tissues and immunosuppression. This study evaluated the phytochemical constituents and assessed the antibacterial activity of the underutilised and lesser-valued part of the cotton fruit tree, specifically its fruit peelings against Aeromonas hydrophila, a fish pathogen in fishes. Crude ethanolic extract (CEE) of cottonfruit peelings was initially assessed for the presence of phytochemicals. The antibacterial activity of the different concentrations of the extract against A. hydrophila was tested using the disc diffusion assay. Phytochemical screening detected phlobatannins, saponins, and coumarins in CEE of cotton fruit peelings. The result of the antibacterial testing showed that all the treatments of the extract inhibit the growth of A. hydrophila, with T4 (100 μg/mL) having the greatest mean zone of inhibition of 14.33 mm. It can be noted that as the concentration of the extract increases, the mean zone of inhibition of all the treatments of the extract also increases. The results showed that phytochemical screening of CEE of cottonfruit peelings revealed the presence of bioactive compounds. It can also be concludedthat it is capable of inhibiting A. hydrophila and thus, it can be an antibacterial agent and has promising potential in improving the current state of the field of aquaculture.

Published

2018-05-18