Anti-proliferative Property of Talinum triangulare (Talilong) Plant Extract on Human Lung (A549) Carcinoma

Abstract

INTRODUCTION

Cancer is a class of diseases characterized by abnormal cell growth that affects healthy cells. It is among the leading causes of morbidity and mortality worldwide and remains a national health priority in the Philippines, occupying the third spot in the leading causes of death in the country after cardiovascular diseases (Philippine Health Statistics, 2009). Talinum triangulare is a small succulent plant that grows wild in many tropical countries. A study conducted by Wang et al. (2014) showed that T. triangulare exhibited antitumor properties on liver cancer cells.This research study aimed to test and compare the anti-proliferative property of different concentrations (0.78125 Âμg/mL; 1.5626 Âμg/mL;3.125 Âμg/mL; 6.25 Âμg/mL; 12.5 Âμg/mL; 25 Âμg/mL; 50 Âμg/mL; 100 Âμg/mL) of T. triangulare plant extract on cancer cell line A549 (human lung carcinoma) by subjecting it to the MTT Assay; and comparing its half maximal inhibitory concentration (IC50) values to the IC50 values of the positive control (Doxorubicin) and the negative control (DMSO).

METHODS

Extraction was done by air-drying T. triangulare leaves, then soaking them in ethanol. This was followed by filtration, then rotary evaporation. This was followed by the MTT Assay. This cytotoxicity assay involved Seeding, Treatment, and Termination. Finally, all materials and equipment were disposed of accordingly. One-way ANOVA was done to statistically analyze the data obtained.

RESULTS

Data collected from the absorbance readings done after the MTT Assay showed the IC50 values of the plant extract and the positive and negative control. For all trials, no linear interpolation was observed from the negative control while low IC50 values were observed from the positive control. The IC50 values obtained from different concentrations of the T. triangulare plant extract were greater than 100 Âμg/mL.

DISCUSSIONS

Results show that the plant extract obtained from T. triangulare was not effective in inhibiting the growth of A549 cells. The One-way ANOVA showed that there is no statistically significant difference among the different concentrations of the T. triangulare plant extract as well as between the experimental set-up and the control groups. This means that the null hypothesis is not rejected and the concentration used in each set-up does not have an effect on the proliferation of A549 cells.