Anti-fungal Activity of Sesbania grandiflora (Katuray) Leaves Extract Against Candida guilliermondii

Abstract

INTRODUCTIONS

esbania grandiflora, commonly known as Katuray, is native to Australia and different countries in Asia like the Philippines. The plants' leaves and bark showed anti-bacterial and anti-fungal properties. The phytochemicals found in Katuray are alkaloids, Flavonoids, Glycosides, Saponins, Tannins, Anthraquinone, Steroid, Pholobatannins, Terpenoids, Carbohydrate, and Protein. Candida guilliermondii is a fungus that is a part of the human microbiota which can be found on the skin. It accounts for 1-3% of the candidema cases. The main objective of this study is to test the antifungal activity of the different concentrations (100%,75%,50%,25%) of Sesbania grandilora leaves extract against the fungus Candida guilliermondii.

METHODS

The Katuray leaves were air dried for 5 days. Then they underwent multi vaporization process in order to produce an extract. The Disk Diffusion Assay was used to test the antifungal activity of Katuray leaves extract against Candida guilliermondii. There is only 1 trial with 3 replicates done in the experimentation. After 7 days, the zone of inhibition was measured in millimeters (mm) using a caliper. One Way ANOVA is the statistical test used to analyze the data gathered.

RESULTS

After 1 week of incubation, the 50% concentration did not inhibit the fungus. The 75% concentration showed inhibition with a diameter of 8 mm on replicate 1 yet did not show inhibition on the other two replicates. The 25% concentration did not show inhibition on the first two replicates yet on replicate 3, the zone of inhibition was similar to the first replicate of the 75% concentration. The 100% concentration showed a trace of inhibition compared to the other concentrations. The gathered data was analyzed using the One-Way ANOVA statistical test. The One -Way ANOVA showed a p-value of 0.595719. The null hypothesis is accepted.

DISCUSSIONS

The 25% and 75% concentration showed the same zone of inhibition of 8 mm on the fungus. The 50% concentration did not inhibit the fungus Candida guilliermondii. The 100% concentration only showed traces of inhibition. Katuray leaves contain phenolic compounds and flavonoids which showed antifungal, antioxidant and antibacterial properties. The null hypothesis is accepted. The study's results will benefit those suffering from Candidiasis. This can serve as a basis of information for other studies related to Candida guilliermondii. To improve the study, it is recommended that future researchers try using other solvents for the extract and try other organisms as the subject.