Hypoglycemic Effects of Angelica Keiskeikoidzumi and Annonanalysing Linn on Musmusculus

Abstract

Ashitaba leaf extractcontains Chalcones (4-hydroxyderricin and xanthoangelol) which has insulin-like activity in vitro and in vivo. On the other hand, Atisleaf extract contains quercetin-3-O-glucoside which regulates alloxan-induced hyperglycemia and lipid peroxidation (LPO)in rats. The study aimed to determine if (1) there is a significant difference between the effects of ashitaba and atis tea on the blood glucose level of the mice after each week of tea administration, and (2) if there is a significant difference in the blood glucose concentrations of mice before and after the fourth week of tea administration. One hundred fifty grams each in powdered form of Ashitaba and Atis were submitted for phytochemical analyses. Based on the analyses, Ashitabacontained sterols, flavonoids, saponins, glycosides and tannins while Atis contained sterols, alkaloids, saponins, glycosides and tannins. The freshly prepared tea extract was administered to ten to twelve week-old alloxan-induced FDA ICR strain male diabetic mice weighing 25 to 30 grams for one month. The mice were made diabetic by intraperitoneal injection of A-7413 Alloxan monohydrate at 180 mg/kg body weight. The mice were grouped into five treatments [T0 (diabetic control) and werefed with standard diet and tap water; T1 was fed with standard diet and 2% Ashitaba tea, T2 was fed with standard diet and 5% Ashitaba tea, T3 was fed with standard diet and 2% Atis tea and T4 was fed with standard diet and 5% Atis tea] having three replications each. Each replication had three mice each for a total of nine mice per treatment. Baseline blood glucose concentrations were estimated prior to the start of the experiment. The study lasted for one month and blood glucose was checked on weekly basis by using a glucometer. The t-test was used for data analyses. Based on the results of the study, the following conclusions were drawn. Blood glucose concentrations of the mice before and after weekly administration of Ashitaba and Atis had decreased due to the flavonoids present in Ashitaba (4-hydroxyderricin and xanthoangelol) and Atis (quercetin-3-O-glucoside). There was a significant difference during the first and third week of glucose measurement in mice after each week of both Ashitaba and Atis tea administration. However, no significant difference was observed during the second and fourth week of glucose measurement in mice. Significant differences were also achieved in testing the blood glucose concentrations of mice before and after the fourth week of tea administration of both Ashitaba and Atis.