Bactericidal Activity of Gold Nanoparticles Formed Using Citrate Synthesis Method against Tilapia (Oreochromis niloticus) Bacterial Pathogen (Aeromonas hydrophila)
Keywords:
aeromonas hydrophila, bactericidal activity, bacterial pathogen, citrate synthesis method, erythromycin, gold nanoparticles, oreochromis niloticus, paper disc assay, mueller hinton agarAbstract
This experimental research synthesized gold nanoparticles using the citrate synthesis method and examined it for its bactericidal activity against Tilapia (Oreochromis niloticus), bacterial pathogen (Aeromonas hydrophila). Three concentrations of 750, 500 and 250 ug/ml of synthesized gold nanoparticles (AuNPs) were subjected to paper disc assay using Mueller Hinton agar against A. hydrophila. Synthesized AuNPs at 750 ug/ml concentration recorded the most effective antibacterial activity against Aeromonas hydrophila among the treatments from the experimental group. However, it was not able to surpass the inhibitory effects of the positive control, erythromycin. Nonetheless, the prevalent antibacterial activity of the gold nanoparticles (AuNP) using the citrate synthesis method showed propitous utilization as a bactericidal mediator. It was confirmed that synthesized AuNPs account for the high antibacterial effectiveness and displayed a great potential in the development of drugs against Aeromonas hydrophila, which is resistant to common antibacterial drugs, and this denotes that it can also be used to treat other fish related bacterial diseases.
