CoaSan: The Utilization of Chitosan Isolated from Bracket Fungi (Trametes sp.) as a Potential Blood Coagulation Agent

Abstract

Crustaceous shells are one of the broadly perceived origins of chitosan; in any case, chitosan can also be found on mushrooms and fungi. This study intended to determine the blood coagulation activity of chitosan extracted from Bracket Fungi (Trametes sp.).The chitosan was extracted from Bracket Fungi using Wiley Mill and was placed in a rotary evaporator at 60 degrees Celsius for two hours. Concentrated extract underwent evaporation using a water bath to be in a semi-solid state. Each level (0.5 g/mL, 1.0 g/mL, and 1.5 g/mL) of chitosan were dissolved on 100 ml of distilled water and 0.2 mL of distilled water (negative control) then were placed on each vial. 0.2 mL of blood from male albino mice was introduced on each vial, one after another. There were two replicates made for each set-up. Each set up was observed until the blood already coagulated. The data were recorded and analyzed using Duncan Multiple Range Test. The 1.50% of chitosan solution had the fastest coagulation time with an average time of 4.73 seconds, while negative control had the slowest coagulation time with 23.63 seconds. Among the blood coagulation time for the chitosan solutions, 1.00% and 1.50% were significantly faster than the 0.50% chitosan solution. Likewise, 1.00% and 1.50% chitosan solutions were significantly faster than the negative control. All the chitosan solution exhibited faster coagulation time compared to the negative control, thus, the results of this research study showed that chitosan extracted from Bracket Fungi mushrooms can induce blood coagulation activity.