The Antibacterial Activity of Bitter Ginger (Zingiber zerumbet) Rhizome Ethanolic Extract Against Escherichia coli ATCC MG1655

Authors

  • Leonoel Panganiban
  • Lovely May Angue
  • Sean Justine Angue

Keywords:

antibiotic-resistance, escherichia coli, the zone of inhibition, antibacterial, zingiber zerumbet (bitter ginger), extraction

Abstract

Antibiotic-resistance is rapidly becoming a major global threat to public health hospitals. In fact, the World Health Organization (WHO) that antibiotic-resistant pathogens infestations will pretence major patient care management issues in the future. Consequently, Escherichia coli causes kidney failure, pneumonia and other infections. This bacteria is becoming resistant in antibiotics. For that reason, this investigatory project aimed to evaluate the antibacterial activity of Zingiber zerumbet (Bitter Ginger). The ability and potential of the ethanolic extract of Z. zerumbet were tested to inhibit known-bacterial pathogen, e. coli. the bacteria were then plated on Trypticase Soy Agar (TSA). Five different concentrations were made consisting of 0:100, 25:75, 50:50, 75:25 and 100:0 ethanol and extract consisting of 9 trials. It was then subjected to standardized bacterial suspension and antibacterial susceptibility was tested. Dipped disk in doxycycline solution will serve as positive control while dipped disks in nystatin solution will serve as a negative control. Zone of inhibition was measured after 24 hours of subjecting. Based on the results gathered, no inhibition was found in different concentrations of ethanolic extract of Zingiber zerumbet (Bitter Ginger). The F-value, 1536.36, is greater than p-value, 4.70E-26. Hence, the null hypothesis was accepted. The research revealed that the ethanolic extract of Zingiber zerumbet (Bitter Ginger) has no ability to inhibit E. coli. One factor affecting the result is the process of making the extract. The rhizome that was bought was fresh which the secondary metabolites can be deteriorate in the process. Medicinal plants are an abundant source of antibacterial molecules. However, other factors affecting the activity needs particular attention. On the other hand, this study will still contribute towards disentanglement new knowledge and can help future researchers in creating natural medicine that can be used as a substitute in hospital-medicine without acquiring negative side effects.

Published

2019-12-18