Antifungal Efficacy of Soap Containing Lagundi Extract (Vitex negundo), Aloe Vera (Aloe barbadensis Miller), Garlic (Allium sativum), and Philippine Lime (Citrus microcarpa) through Lab Testing


  • Vincent Bob Arcilla General de Jesus College
  • Joshua Junia General de Jesus College
  • John Aaron Mercado General de Jesus College
  • Sean Kian Cabrito General de Jesus College
  • Jann Michaela Jaraba General de Jesus College
  • Wiljanh de Belen General de Jesus College
  • Ronnel Francisco General de Jesus College


Pharmacology & pharmaceutical medicine, Laboratory testing, Philippine Lime, Antifungal, efficacy, Soap, Lagundi extract, Aloe Vera, Garlic


Fungal infections are a global health concern affecting a significant population. Antifungal soaps are commonly used to prevent and treat these infections. However, there is a growing demand for alternative soap formulations that are effective, affordable, and accessible to a broader population. This study aimed to develop an experimental soap using natural ingredients and evaluate its efficacy in inhibiting fungal growth and spread. The experimental soap was formulated using palm oil, canola oil, coconut oil, lye, water, and plant extracts, including garlic, Aloe Vera, Philippine Lime (Calamansi), and Lagundi. Two versions of the soap were prepared: solid and liquid. The formulation followed specific ratios of oils to lye, with precise measurements of each ingredient. Aspergillus Niger, a common food mold, was used for observation to assess the antifungal efficacy. The effects of the soap were monitored over six days, with observations recorded every three days.

The spread of the fungi was visually evaluated by comparing the areas treated with soap to untreated areas. The experimental soap, both in its solid and liquid forms, demonstrated antifungal properties. The solid soap showed comparable effectiveness to sulfur, a widely used antifungal soap. However, there was a slight variation in inhibiting fungal growth and spread, with the experimental soap being approximately 3% to 10% less effective than sulfur soap. The liquid soap exhibited lower efficacy than sulfur soap, with a margin of around 10% to 15% less effectiveness. In some instances, fungal infections were observed in areas treated with liquid soap, suggesting limitations in inhibiting fungal growth.

In conclusion, the experimental soap showed promising results in inhibiting fungal growth and spread, although slightly less effective than sulfur soap. This study contributes to the understanding of alternative soap formulations for fungal infections. However, further research and development are necessary to address the observed limitations, such as improving the soap's spread and addressing fungal infections in specific areas. These findings provide valuable insights for future researchers and entrepreneurs in developing antifungal soaps. They underscore the importance of ongoing investigation and refinement of soap formulations to enhance their antifungal properties and potency.