Bacteriological Analysis of Osorio's Spring Water with Utilization of Filtration and Boiling Process

Abstract

INTRODUCTION

Water is an essential part of life. It is used in daily activities; drinking, cooking, etc. However, as human use this, they are not aware of unseen bacteria such as Escherichia Coli, Fecal Coliform, Coliform, and Heterotrophs. Thus, examining and identifying the bacteria present in water is very important for human's safety. In this experimental research, the bacteria count in the spring water of Barangay Osorio, Trece Martires City was determined and indicated if it is safe to drink. Moreover, with the use of filtration and boiling process, this study showed the difference of bacteria count between the filtered and unfiltered spring water.

METHODS

This research study used quantitative method experimental research design as it aims to analyze the bacteria count and to determine the effectiveness in lessening of these bacteria through filtration and boiling process. The samples were gathered at Barangay Osorio, Trece Martires City. Researchers prepared several materials, followed some methods in conducting the research, and used the standard values adopted from Philippine National Standards for Drinking-Water (PNSDW) to score and interpret the data. Finally, Independent Paired t-test was utilized for statistical treatment.

RESULTS

Using multiple tube fermentation technique for both E.coli and Potability Test (Fecal Coliform and Coliform); pour plate HPC, the results showed that, before utilizing filtration and boiling process in Osorio's spring water, the samples had a count of greater than 8.0 MPN/100ml for both E. coli and Potability Test in bottle 1 and 6,067 CFU/100ml for the HPC Test; greater than 8.0 MPN/100ml for both E. coli and Potability Test and 5,967 CFU/100ml for the HPC Test in bottle 2. Meanwhile, after it was filtered and boiled, the samples had a count of less than 1.1 MPN/100ml for both E. coli and Potability Test in bottle 1.1 and 510 CFU/100ml for the HPC Test; less than 1.1 MPN/100ml for both E. coli and Potability Test and 478 CFU/100ml for the HPC Test in bottle 2.1.

DISCUSSIONS

There is a significant difference in the count of E. Coli, Fecal Coliform, and Heterotrophs present in the water sample before and after filtration and boiling. The study found out that water samples that were filtered and boiled shows a huge decrease in bacteria count. Thus, the researchers concluded that the elimination of bacteria in the water could be possible through the utilization of boiling and filtration process.