Susceptibility of the Methicillin-Resistant Staphylococcus aureus in the Biosynthesized Silver Nanoparticles by Bacillus subtilis

Abstract

INTRODUCTION

Due to the increase of multidrug-resistant bacteria, the discovery of new sources of antibiotics has seen an upsurge. In addition to this, the resurgence of Methicillin-resistant Staphylococcus aureus (MRSA) has created the necessity for studies directed towards the development of new antimicrobials.

METHODS

Nanoparticles have already made its way to be utilized in making a bacterium a potential alternative for antibiotics. From these precepts, this research study aimed to use Biosynthesized Silver Nanoparticles (AgNP) by Bacillus subtilis in inhibiting MRSA. Cultures of B. subtilis were obtained, broth and agar were prepared, B. subtlis were inoculated, centrifuged, and subjected to MRSA through agar well diffusion method; and the zone of inhibition was obtained.

RESULTS

Findings showed that the greatest zone of inhibition is the positive control with an average diameter of 24.33mm, followed by the negative control (14.67mm) and the biosynthesized AgNP by B. subtilis (14.17mm) subjected in MRSA.

DISCUSSIONS

Based on the results presented through the zone of inhibition, there is a significant difference in the positive, negative, and silver nanoparticles. The description was also supported by the statistical analysis using One-way ANOVA. The F was 74.83122, while the computed F crit was 3.68232. In relation to the hypothesis, since the F was greater than the F crit, the null hypothesis was rejected. With this, it can be delineated that biosynthesized AgNP by B. subtilis can be used as an alternative against MRSA.