Insulin Mimetic Ability of Hagonoy (Chromolaena Odorata) Against Induced Diabetic Swiss Mice (Mus Musculus)

Abstract

INTRODUCTION

Diabetes is one of the widespread diseases in the world today. Compared to communicable diseases such as malaria and cholera, diabetes is a disease that links with one's lifestyle or the way how people live their life. It is also associated with other lifestyle diseases like overweight and obesity. Currently, diabetic people rely on insulin and synthetic drugs for treatment. However, expensive price of these pharmaceutical drugs motivated many researchers to determine and evaluate the antidiabetic potential and insulin mimetic ability of some plants that can serve as a cheaper alternative treatment for diabetes. This investigatory project aims to determine the insulin mimetic ability of Hagonoy (Chromolaena odorata) leaves extract against Induced Diabetic Swiss Mice (Mus musculus).

METHODS

The experimental design employed in this study wasa five-group parallel design wherein a positive, negative control and three (3) treatments were manipulated. Each treatment was replicated three times for a total of 15 experimental units. The assigning of replications to the experimental units was done at Complete Randomized Design (CRD). The plant material was gathered from the locality and extracted through decoction. Three (3) sets of Hagonoy leaves extract solution with 5%, 3% and 1% percentage by volume were prepared by the researcher. The solutions were induced through intravenous injection. Fifteen (15) Swiss mice were assigned into 5 groups and were acclimatized for 7 days and put into 30 days diet dysregulation that aimed to increase the blood glucose level. The blood samples were collected and glucose level was measured before and after the treatment using a glucometer. The gathered data were treated statistically using Paired T-test and ANOVA.

RESULTS

The results revealed that there is no significant difference in the fasting glucose level after the induced insulin mimetic treatment of Hagonoy (Chromolaena odorata) aqueous leaves extract and the insulin mimetic commercial drug metformin. It also showed that there is a significant difference on the fasting glucose level after the induced insulin mimetic treatment of Hagonoy (Chromolaena odorata) aqueous leaves extract and the negative control (untreated).

DISCUSSIONS

Hagonoy is considered as one of the invasive plants and a noxious weed for it has characteristics that are harmful to human, animals and to the environment. The detection of insulin mimetic ability of Hagonoy can lead to the development of pharmaceutical drugs.