Diabetest: Paper-Based Microfulidics Test for the Amount of Glucose and Ketone Present in the Newly Excreted Urine

Abstract

INTRODUCTION

Despite the tremendous advancement in the medical field, millions of people living with diabetes are not aware of their own body condition as stated by National Diabetes Statistics Report. In type 1 Diabetes, the body resort in burning fats to produce energy resulting to a high concentration of ketone in the urine while type 2 is simply the presence of glucose.

METHODS

Out of the different research design, the control group design was performed in which there was one control group and one experimental group observed. The researcher utilized the glucose oxidase method since it was proven to be the most glucose concentration sensitive. Using Legal's Test and Glucose Oxidation Method the study was able to produce a paper-based microfluidics testing strip.

RESULTS

Results showed that 0.01% of glucose in one Liter of urine can be determined using the product while ketone can be detected once it exceeded 0.6 mmol/L. On the other hand, the theoretical ketone detected in the colorimetric table is between 4.0-6.0 mmol/L while the laboratory results showed 4.67mmol/L, both lies in the same range of being positively moderated. Hexokinase method indicated 124 mg/100 mL while the testing strip exhibited 120-125 mg/100 mL.

DISCUSSIONS

After several solution enhancements, the injected liquid to the stiff paper was then tested via a sample of a newly excreted urine from both Type 1 and Type 2 patients. The results from the colorimetric table were compared to test center analysis. From the obtained results and certificates released by various laboratories inside and outside the Philippines giving proof of the product's analysis passing and accuracy, the researcher now concludes that the produced strips are effective in giving pre-diagnosis for diabetes.